Biogenesis Read Online Free PDF

frequent searches on a small scale with children in tow. Putting aside the validity of Dr. Akedera’s conjecture, it is certainly an interesting approach to take, and if it yields results, the validity issue will have been settled after a manner. In addition, subsequent investigations by the Mutsumi Club determined that the families appearing in the photos were relatively close kin, but this may simply have been a coincidence.
    So much for our digression. At the suggestion of the Mutsumi Club, experimentation on the two mice in captivity was considered, but the city council and committee members’ consent proved nearly impossible to obtain on experiments outside the established protocols for species preservation. That avenue had to be abandoned.
    Immediately afterwards, major set-up work at the Species Preservation Center got underway. Under Dr. Sakakibara’s ministrations, a compact clean bench, thermostatically controlled tanks, five incubators, an electronic scale, an autoclave, compressed gas, a phase-contrast stereomicroscope, disposable plastic products and more were brought into the pathology lab. In addition, five refrigerators, requisitioned from the town hall and other public facilities with the assistance of the Mutsumi Club, were placed in the adjacent room, and in them went the reagents and serum kits that had been delivered that afternoon.
    In truth, this was not the first time an attempt had been made to culture cells from the winged mouse. The first researcher, Dr. Ishikawa, had already tried (using a mouse caught at Indian Peak that subsequently died). He had asked an acquaintance of his, the clinical lab technician Mitsuo Miura, to perform a primary culture of epidermal fibroblasts, but subsequent cultures had failed and it was left at that. Since the center had lacked the apparatuses for culturing cells, Mr. Miura had packed a clean box and a simple incubator intothe back of a van –– sitting with them to guard against shaking –– while his wife Tsutako had driven them from a lab in Sapporo to Fukagawa.
    In spite of such efforts, Dr. Ishikawa made no mention of it during his academic presentation.
    Dr. Akedera had conducted a phone interview with Mr. Miura prior to departing for Hokkaido, but why the attempt failed remained unclear. Even after a detailed examination, no problems came to light on the basic points, such as the time between death and culturing, or the procedure itself.
    Either the fetal bovine serum had not been suitable for the cells, or the conditions of the culturing medium had been problematic, or alternatively, the two factors together had been an infelicitous combination, Dr. Akedera concluded in the end. Cells can die off when a culture is contaminated with a resistant strain of bacteria, and perhaps such a possibility merited adequate consideration given how they had gone about it. De facto countermeasures, however, were already in place.
    The work began by heating and deactivating as many as fifty types of serum and dissolving the glutamine. All sorts of combinations of the serums with thirty types of culturing medium, thirteen types of antibiotics and antifungal agents, growth factors, transferrin, insulin, etc., were created and subdivided in the clean bench. The effort lasted until after midnight, but ultimately nearly three hundred culturing media were stored in the refrigerators.
    It may be that major discoveries owe to coincidence and luck regardless of the epoch. Good fortune smiled on Dr. Akedera, too, as if a strange destiny were guiding him.
    Somehow, the content of a phone interview that had been conducted with Dr. Ishikawa’s wife was brought to Dr. Akedera’s attention only that night, via Dr. Sakakibara.
    “That day, I had a phone call from the Mutsumi Club asking about the winged mouse. It might havebeen because the gas cylinder was being changed out at the time, but … the question concerned shining, so I replied that I had no idea what was inside the